Amphibian biodiversity is declining worldwide, and accessible cryopreservation tools are needed to support germplasm repositories, especially in low-resource settings. We developed a platform that integrates 3D-printed open-hardware components with gelatin capsules as containers for amphibian sperm cryopreservation, using Xenopus laevis as a model.

Rodrigues de Freitas, T., Streit, D. P., Jr, Harmon, E., dos Santos Teixeira, N., Lisboa Benato, J., de Souza França, T., Koch, J. C., & Tiersch, T. R. (2026). An open-hardware platform for conservation of amphibian genetic resources based on gelatin capsules. iScience, 29(8), 117103.

Precise control of microscale object rotation is essential for numerous biomedical and microelectromechanical applications. For example, somatic cell nuclear transfer for aquatic biomedical models such as zebrafish faces significant technical challenges, particularly in egg trapping and alignment of an injection needle with the micropyle. In this study, we developed a 3D resin-printed microdevice to achieve frequency-selective electrorotation of dielectric microspheres using a quadrupole electrode configuration driven by phase-shifted alternating current (AC).

Hosseini, S., Norouzi, M., Cibelli, J., Koch, J. C., Monroe, W. T., Veronis, G., Tiersch, T. R., Xu, J., & Liu, Y. (2025). A 3D-printed microdevice for dielectrophoretic torque–driven rotation of dielectric microspheres to support development of manipulation systems for zebrafish eggs. Journal of Micromechanics and Microengineering: Structures, Devices, and Systems, 35(12), 125005.